Title | High-quality genomic DNA extraction from formalin-fixed and paraffin-embedded samples deparaffinized using mineral oil. |
Publication Type | Journal Article |
Year of Publication | 2009 |
Authors | Lin, J, Kennedy, SH, Svarovsky, T, Rogers, J, Kemnitz, JW, Xu, A, Zondervan, KT |
Journal | Anal Biochem |
Volume | 395 |
Issue | 2 |
Pagination | 265-7 |
Date Published | 2009 Dec 15 |
ISSN | 1096-0309 |
Keywords | DNA, Fixatives, Formaldehyde, Mineral Oil, Paraffin Embedding, Polymerase Chain Reaction, Time Factors |
Abstract | Extracting DNA from formalin-fixed and paraffin-embedded (FFPE) tissue remains a challenge, despite numerous attempts to develop a more effective method. Polymerase chain reaction (PCR) success rates with DNA extracted using current methods remain low. We extracted DNA from 140 long-term archived FFPE samples using a simple but effective deparaffinization method, removing the wax with mineral oil, and a commercially available DNA extraction kit. DNA quality was subsequently tested in a genotyping experiment with 14 microsatellite markers. High-quality DNA was obtained with a mean PCR success rate of 97% (range: 88-100%) across markers. The results suggested that DNA extracted using this novel method is likely to be suitable for genetic studies involving DNA fragments <200 bp.
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DOI | 10.1016/j.ab.2009.08.016 |
Alternate Journal | Anal Biochem |
PubMed ID | 19698695 |
PubMed Central ID | PMC2764035 |
Grant List | 085235 / WT_ / Wellcome Trust / United Kingdom |