Direct selection of human genomic loci by microarray hybridization.

Title	Direct selection of human genomic loci by microarray hybridization.
Publication Type	Journal Article
Year of Publication	2007
Authors	Albert, TJ, Molla, MN, Muzny, DM, Nazareth, L, Wheeler, D, Song, X, Richmond, TA, Middle, CM, Rodesch, MJ, Packard, CJ, Weinstock, GM, Gibbs, RA
Journal	Nat Methods
Volume	4
Issue	11
Pagination	903-5
Date Published	2007 Nov
ISSN	1548-7091
Keywords	BRCA1 Protein, Cell Line, Tumor, Gene Library, Genome, Human, Humans, Nucleic Acid Hybridization, Oligodeoxyribonucleotides, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Sequence Analysis, DNA
Abstract	We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.
DOI	10.1038/nmeth1111
Alternate Journal	Nat Methods
PubMed ID	17934467
Grant List	R21HG004553 / HG / NHGRI NIH HHS / United States U54 HG 003273 / HG / NHGRI NIH HHS / United States